Nutrient Agar - ReadyMED 1kg

Description

Principle:- Nutrient Agar is basic medium used for cultivation and maintenance of fastidious microorganisms, by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing. Nutrient Agar is composed of vegetable peptones to avoid Transmissible Spongiform Encephalopathy and Bovine Spongiform Encephalopathy risk associated with animal peptone. It is consist of peptone, extract and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium. The media can be fortified with different biological fluids such as horse or sheep blood, serum; egg yolk etc. makes it suitable for the cultivation of related fastidious organisms. Directions: Dissolve 28.00 grams in 1000 ml distilled water. Sterilize by autoclaving at 15 lbs. pressure (121 °C) for 15 min, cool it to 42-45 °C. If desired, the medium can be enriched with 5-10% blood or other biological fluids. Nutrient Agar distribute aseptically in desired and allow to solidify. Ensure complete solidification and inoculate test sample aseptically.